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Hu, J. G., Sun, B. Q., Bizounok, M., Hatcher, M. E., Lansing, J. C., Raap, J., Verdegem, P.J.E., Lugtenburg, J., Griffin, R. G., and Herzfeld, J.. 1998. Early and Late Intermediates in the Bacteriorhodopsin Photocycle: A Solid-State NMR Study. Biochem. 37: 8088-8096. Full Article

Solid-state deuterium NMR is used to investigate perturbations of the local, internal dynamics in the EcoRI restriction binding site, -GAATTC- induced by cytidine methylation. Methylation of the cytidine base in this sequence is known to suppress hydrolysis by the EcoRI restriction enzyme. Previous solid-state deuterium NMR studies have detected large amplitude motions of the phosphate-sugar backbone at the AT-CG junction of the unmethylated DNA sequence. This study shows that methylation of the cytidine base in a CpG dinucleotide reduces the amplitudes of motions of the phosphate-sugar backbone. These observations suggest a direct link between suppression of the amplitudes of localized, internal motions of the sugar-phosphate backbone of the DNA and inhibition of restriction enzyme cleavage.

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