Emily Wiley
Professor of Biology
Email: ewiley@kecksci.claremont.edu
Office: Keck Science Center 210
Phone: 909-607-9698
Office Hours:
Web Site: http://sites.google.com/site/wileylabs/Home
Educational Background:
B.A., Western Washington University
Ph.D., University of Washington
Postdoc, University of Rochester; Fred Hutchinson Cancer Research Center
Courses Taught:
Molecular Biology (Bio170L) Molecular Seminar (Bio173L) Accelerated Integrated Science Sequence (AISS) Introductory Biological Chemistry (IBC) (Bio40) Drugs and Molecular Medicine (Bio144) Biochemistry (Bio177) Introductory Biology (cell/molecular/genetics/physiology) and Introductory Laboratory (Bio43L) Genetic Engineering and Biotechnology (Bio84L)
Research Interests:
My lab studies the relationships between histone modifications, chromatin structure, the control of gene expression, and nuclear differentiation using the protozoan Tetrahymena thermophila as a model system. Most of our work focuses on exploring the role of histone deacetylases in chromatin dynamics and heterochromatin assembly, and histone clipping mechanisms. EXTERNAL GRANTS: 2014-18 NSF Improving Undergraduate Science Education (IUSE) Award: "The Ciliate Genomics Consortium Model for Sustainable Teaching-Research Integration" 2012-17 NSF Sub-Award: "Genomics of Tetrahymena" 2006-13 NSF CAREER Award: "Investigating Chromatin Assembly Pathways Through Histone Deacetylases"
Thesis Topics:
Cellular localization of histone deacetylase enzymes through GFP-tagging;
Engineering gene knockout constructs of chromatin modification enzymes, generation of knockout cell lines, and phenotype characterization of mutant cells;
Identification of protein complexes that modify chromatin;
Mechanistic roles for histone deacetylase enzymes in the control of gene expression and other cellular processes
Selected Publications List: Click to open new window.
1.   Wiley, E.A. and Chalker, D . (2016). A community model for course-based student research that advances faculty scholarship. CUR Quarterly   37(2): . Article
2.   Yale, K., Neuman, M., Bulley, E., Tackett, A., Chait, B.T., Wiley, E.A . (2016). Phosphorylation-dependent targeting of Tetrahymena HP1 to condensed chromatin. mSphere 4   4: . Article
3.   Wiley, E.A. and Stover, N . (2014). Immediate dissemination of student discoveries to a model organism database enhances classroom-based research experiences. CBE Life Sci. Educ   13(1): 131-8.
4.   Smith, J.J., Wiley, E.A., Cassidy-Hanley, D . (2012). Tetrahymena in the Classroom. Methods in Cell Biology   109: 411-30.
5.   Slade, KM., Freggiaro, S., Cottrell, K.A., Smith, J.J., and Wiley, E.A . (2011). Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena. BMC Cell Biology 12(1):40-54   12(1): 40-54.
6.   Sean R. Gallagher and Emily A. Wiley . (2008). Current Protocols: Essential Laboratory Techniques. (Gallagher, S.R. and Wiley, E.A, eds.) Wiley and Sons, Inc., New Jersey : .
7.   Coyne, R.S., Thiagarajan, M., Jones, K.M., Wortman, J.R., Tallon, L.J., Haas, B.J., Cassidy-Hanley, D.M., Wiley, E.A., et al . (2008). Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure. BMC Genomics   9: 562-579.
8.   Smith, J., Torigoe, S., Maxson, J., Fish, L., and Wiley, E.A . (2008). A class II HDAC deacetylates newly-synthesized histones in Tetrahymena. Eukaryotic Cell   7(3): 471-482.
9.   Parker, K., Maxson, J., Mooney, A., and Wiley, E.A . (2007). Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila. Eukaryotic Cell   6: 1913-1924.
10.   Wiley, E.A., Myers, T., Parker, K., Braun, T., Yao, M.-C . (2005). The class I histone deacetylase Thd1p affects nuclear integrity in Tetrahymena thermophila. Eukaryotic Cell   4: 981-990. Abstract
11.   Wiley, E.A., Mizzen, C., Allis, C.D . (2000). Isolation and characterization of in vivo modified histones and an activity gel assay for identification of histone acetyltransferases. . Methods in Cell Biology: Tetrahymena thermophila. (James Forney and David Asai, eds.) Academic Press 62: 379-394.
12.   Wiley, E.A., Ohba, R., Yao, M.-C., Allis, C.D . (2000). Developmentally regulated Rpd3p homolog specific to the transcriptionally active macronucleus of vegetative Tetrahymena thermophila. Molecular and Cellular Biology   23: 8319-8328. Abstract
13.   Huang, H., Smothers, J.F., Wiley, E.A., Allis, C.D . (1999). A nonessential HP1-like protein affects starvation-induced assembly of condensed chromatin and gene expression in macronuclei of Tetrahymena thermophila. Molecular and Cellular Biology   19: 3624-3634. Abstract
14.   Huang, H., Wiley, E.A., Lending, C.R., Allis, C.D . (1998). An HP1-like protein is missing from transcriptionally silent micronuclei of Tetrahymena.. Proceedings of the National Academy of Science   95: 13624-13629. Abstract