Selected Publications for: David Sadava

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1.   Sadava, D., Heller, C., Orians, G., Purves, W. and Hillis, D.. (2008). .  Sinauer Associates and WH Freeman and Co. : . Article



2.   D. Sadava, E. Whitlock, S. E. Kane. (2007). The green tea polyphenol, epigallocatechin-3-gallate inhibits telomerase and induces apoptosis in drug-resistant lung cancer cells. Biochemical and Biophysical Research Communications   360: 233-237.



3.   D. Sadava and W, Hamman. (2007). RNA interference against MDR1 but not MRP1 reverses drug resistance in human small-cell lung cancer cells. Journal of Cancer Molecules   3: 91-94.



4.   D Sadava. (2006). "The story of PC-SPES and prostate cancer". Textbook of Complementary and Alternative Medicine C. Yuan Taylor and Francis Medical Books : .



5.   D Sadava and J Winesburg. (2005). Contaminants are not responsible for cytotoxicity of PC-SPES in human small-cell lung carcinoma cells”. Cancer letters   220: 171-175.



6.   W. Purves, G. Orians, C. Heller, D. Sadava. (2004). Life: The Science of Biology.  Sinauer Associates- WH Freeman 6: .



7.   M. Chrispeels and D, Sadava. (2003). Plants, Genes and Crop Biotechnology.  ASPB./Jones and Bartlett : .



8.   R. E. Schwarz, C. Donohue, S. Kane, D. Sadava. (2003). Pancreatic cancer toxicity mediated by Chinese herbs SPES and PC-SPES: Implications for monotherapy and combination treatment. Cancer Letters   189: 59-68. Abstract Article


Abstract: One of the greatest challenges in the treatment of pancreatic cancer remains its inherent lack of beneficial response to cytotoxic chemotherapy. Chinese herbal extracts have been widely used for the treatment of various cancers, but objective information on their efficacy in pancreatic cancer is lacking. Eight human pancreatic cancer cell lines (MIA, Panc-1, BxPC, ASPC, HS-766T, CaPan-2, CFPAC, and HTB-147) were studied for in vitro susceptibility to ethanol extracts of SPES and PC-SPES, two quality-controlled, dried, encapsulated supplements of 15 and eight Chinese herbs, respectively. Resulting toxicities, alone and in combination with doxorubicin or gemcitabine, were analyzed by [3H]thymidine incorporation or sulforhodamine B staining, colony formation, and TUNEL flow cytometry assays. Combination toxicity mechanisms were calculated by the combination index method of Chou and Talalay. In all cell lines, there was dose-dependent inhibition of proliferation. By [3H]thymidine incorporation assay, 50% growth inhibition after 48 h continuous exposure (IC50) occurred at concentrations of 0.2–0.8 l/ml SPES and 0.4–1.3 l/ml PC-SPES. Growth inhibition was accompanied by a significant enhancement of the TUNEL-positive apoptotic fraction of all cell lines after treatment with either extract. After treatment with PC-SPES, the cell lines consistently displayed a G2 cell cycle block; SPES induced an increase in S phase, with a smaller impact on G2. When added at a concentration of 0.2 l/ml (~IC20), both extracts enhanced Panc-1 cell killing mediated by doxorubicin, with an average decrease in the corresponding IC50 of 33% (range 11–62%). Combination effects with either extract appeared to be antagonistic in the case of gemcitabine and additive to mildly synergistic in the case of doxorubicin. Both SPES and PC-SPES exhibited significant toxicity in pancreatic cancer cells, mediated via induction of apoptosis. Both mixtures should be evaluated for their in vivo and clinical therapeutic utility as monotherapy agents against pancreatic cancer. SPES could possibly be combined with cell cycle-independent cytotoxic drugs. Due to a consistent G2 blocking pattern, PC-SPES may prove useful as a radiation sensitizer.


9.   J. Ahn, M. Zhan, M. Pang, J. Ding, S. Kane, D. Sadava. (2002). Effects of four Chinese herbal extracts on drug-sensitive and drug-resistant human small-cell lung carcinoma cells. Cancer Chemotherapy Pharmacol   49: 261-266.



10.   C. Lin, T. Phillips, S. Kane, D. Sadava. (2002). Artemisinin and transferrin: Use in overcoming drug resistance in small cell lung cancer cells. Cancer Letters   172: 151-156.



11.   A. Coleman, S. Kane. (2002). Liposomal daunorubicin overcomes multi-drug resistance in human breast cancer, ovarian cancer and small cell lung cancer cells. J. Lipos. Res.   12: 301-309.



12.   W. Purves, G. Orians, C. Heller, D. Sadava. (2001). Life: The Science of Biology.  Sinauer Associates. W. H. Freeman 6: .



13.   W. Purves, G. Orians, C. Heller, D. Sadava. (2001). Life: The Science of Biology.  Sinauer Associates - W. H. Freeman 5: .



14.   K. Heidel, A. McMurtray, K. Scanlon, D. Sadava. (1998). Neuron-specific enolase and thymidine kinase: Markers for multi-drug resistance and its reversal in human small-cell lung cancer cells. J. Tumor Marker Oncol.   13: 49-52.



15.   T. Remer, K. Peterson, D. Sadava. (1997). Hyperplasia, hyperproliferation and decreased migration rate of colonic epithelial cells in mice fed a diet deficient in vitamin D. Bio. Cell   87: 113-116. Abstract Article


Abstract: Low serum levels of vitamin D metabolites have been associated with an increased risk for colon cancer. To investigate the effects of vitamin D deficiency on the colon, 4-week-old mice were fed a diet either containing (100000 IU/kg diet) or lacking this vitamin for 3 weeks. Food consumption and body weight gain were similar in both groups. Following injection with 3H-thymidine to label dividing cells, cellular proliferation and migration up the colonic crypt were determined autoradiographically. Although overall crypt lengths were similar in both groups, there was hyperplasia and hyperproliferation in crypts of the deficient animals. Also, their epithelial cells migrated up the crypt at a significantly slower rate (maximum 0.78 m/h) than did those from control mice (1.42 m/h). There was no difference in cellularity, proliferation or migration in duodenal epithelium. These results indicate that vitamin D deficiency significantly alters colonic but not duodenal epithelial cells.


16.   D. Alonso, H. Hong, D. Pettit-Barrett, D. Sadava. (1997). Effect of methadone addiction on glucose metabolism in rats. Gen. Pharmacol   28: 27-29. Abstract


Abstract: Female albino rats were exposed to methadone over a 35-day period by addition of the drug in their drinking water. The final dose of the drug was 1.8 mg/kg body weight per day. After this period, the drug was withdrawn from some animals for 30 days (postexposure). Compared to unexposed controls, serum glucose levels rose during exposure and returned to control levels postexposure. Oral glucose tolerance tests showed impairment in 35-day drug-exposed animals compared to controls and postexposure. The activities of three key enzymes of glycolysis and three key enzymes of gluconeogenesis were measured in liver during and at the end of the exposure period, as well as postexposure. Compared to unexposed controls and postexposure, specific activities of two glycolytic enzymes in livers of exposed animals—hexokinase and phosphofructokinase 1—were significantly reduced, whereas the activity of a third glycolytic enzyme—pyruvate kinase—was unchanged. The specific activities of two gluconeogenic enzymes—glucose-6-phosphatase and fructose-1,6-biphosphatase—were significantly elevated in the drug-exposed animals compared to controls, whereas the activity of a third enzyme—phosphoenolpyruvate carboxykinase—was unchanged. These data indicate that methadone addiction produces a metabolic state similar to insulin-resistant diabetes.


17.   A. McMurtray, K. Heidel, D. Sadava. (1997). Markers for multi-drug resistance and its reversal in human small-cell lung cancer cells. J. Tumor Marker Oncol.   87: 113-116.



18.   T. Castillo, D. Sadava. (1996). Growth of human small-cell lung cancer cells in AIM-V, a serum-free medium. Focus   18: 59-60.



19.   P.W. Luo, J. Casper, D. Sadava. (1996). Induction of renal damage to rats by a diet deficient in antioxidents. Nutr. Res.   16: 1607-1612.



20.   H. Kijima, H. Ishida, L. Xia, K. J. Scanlon, D. Sadava. (1995). The role of oncogenes in drug-resistant human carcinoma cells. AACR Proceedings   36: 343.



21.   M. Chrispeels, D. Sadava. (1994). Plants, Genes and Agriculture.  Jones and Bartlett, Publishers : .



22.   J. Tsai, S. Gould, D. Sadava. (1994). Imaging of the P-glycoprotein in normal and MDR small cell lung carcinoma cells with the atomic force microscope. Proc. Mtg. Microscopy Society (G. Bailey and A. Garrett-Reid, eds.)  : 1074-5.



23.   D. Sadava. (1993). Cell Biology: Organelle Structure and Function.  Jones and Bartlett Publishers : .



24.   P. Frykman, E. Harris, D. Majerus, J. Mustard, B. Bernard, D. Sadava. (1992). Development of enzymes of glycolysis and gluconeogenesis in human fetal liver. Biol. Neonate   62: 89-95.



25.   B. Bernard, D. Sadava. (1990). Transition from cytosolic to mitochondrial thymidine kinase during development in human fetal tissues. Life Sci.   47: 2359-2394.



26.   D. Sutcliffe, D. Sadava. (1988). The effects of maternal hyperphenylalaninemia on learning in mature rats. Life Sci.   43: 1119-1123.



27.   K. Moore, D. Sadava. (1987). Glycerol metabolism in higher plants: glycerol kinase. Biochem. Biophys. Res. Commun   143: 977-983.



28.   M. Depper, M. Gilbert, B. Bernard, E. McCabe, D. Sadava. (1987). Development of enzymes of glycerol metabolism in human fetal liver. Biol Neonate   52: 26-33.



29.   D. Sadava. (1987). Increased cellular turnover rate of colonic but not duodenal epithelium in mice fed a high fiber diet. Med. Sci. Res.   15: 583-584.



30.   B. Mack, D. Sadava. (1986). Effect of methadone addiction on cyclic nucleotide levels in regions of rat brain. Life Science   39: 477-481.



31.   K. Wilmington, D. Sadava. (1984). Tricyclic antidepressant drugs affect histamine receptors in human leukocytes.. Life Science   35: 2545-2548.



32.   H. McKelvey, E. McCabe, C. Rose, D. Sadava. (1982). Glycerol kinase deficiency: enzyme kinetics and fibroblast hybridization. J. Inherit. Metab. Dis.   5: 177-182.



33.   D. Watumull, K. Sanders, K. Downey, D. Sadava. (1982). The affect of vitamin C on the rapid induction of aortic changes in rabbits.. J. Nutr. Sci.   28: 85-92.



34.   E. McCabe, W. Seltzer, R. Hill, D. Sadava. (1982). Glycerol kinase: developmental biochemistry in man. Pediatric Research   16: 298.



35.   B. Bernard, K. Elliott, D. Sadava. (1980). Multiple forms of thymidine kinase in serum of premature infants. Biol. Neonate.   38: 287-290.



36.   . (1978). Synthesis and secretion of cell wall glycoproteins in carrot root disks. Biochemistry of Wounded Plant Tissues (G. Kahl, W. de Gruyter ed.)  : 85-102.



37.   M. Chrispeels, D. Sadava. (1978). Extensin: role in the cessation of elongation in excised pea epicotyls. Develop. Biol.   30: 49-57.



38.   M. Chrispeels, D. Sadava. (1977). Plants, Food and People.  W.H. Freeman and Co. : .



39.   T. Field, A. McNelly, D. Sadava. (1977). Effect of maternal methadone addiction on offspring in rats. Arch. Int. Pharm. Ther.   228: 300-305.



40.   B. Bernard, P. Beaupre, K. Elliott, B. Preston, D. Sadava. (1977). Multiple forms of thymidine kinase during human development. Biol. Neonate.   228: 300-305.



41.   B. Bernard, P. Beaupre, K. Elliott, B. Preston, D. Sadava. (1977). Multiple forms of thymidine kinase during human development. Biol. Neonate.   31: 225-228.



42.   B. Volcani, D. Sadava. (1977). Biochemistry of silica shell formation in diatoms. Planta   135: 7-11.



43.   M. Schoenfield, D. Sadava. (1976). Effect of methadone addiction on N demethylation activity of rat liver microsomes. Arch. Int. Pharm. Ther.   219: 347-350.



44.   D. Sadava. (1976). Attitudes toward science of nonscience major undergraduates . J. Res. Sci. Tchg   13: 69-84.



45.   M. Sloane, D. Sadava. (1975). Auxin, carbon dioxide and hydrogen ions. Nature   257: 68.



46.   Y. Cho, M. Chrispeels, D. Sadava. (1974). Enhancement of extensin biosynthesis in aging disks of carrot storage tissue.. J. Exp. Botany   25: 1157 1166.



47.   M. Flaherty, D. Sadava. (1974). A rat model for methadone addiction. Arch. Int. Pharm. Ther.   212: 103-108.



48.   S. Ferronato, D. Thomas, D. Sadava. (1974). Preferences for handedness, arm folding, and hand clasping in families. Human Heredity   24: 345-352.



49.   D. Sadava, M. Chrispeels. (1974). Synthesis and secretion of proteins in plant cells. Macromolecules Regulating Growth and Development (E. Hay ed.) Academic Press : 131-152.



50.   D. Sadava. (1973). Biogenesis of cell wall protein. Biogenesis of Plant Cell Wall Polysaccharides (F. Loewus ed.) Academic Press : 165-175.



51.   F. Walker, M. Chrispeels, D. Sadava. (1973). Extensin: synthesis and accumulation in growing pea epicotyls.. Develop. Biol.   30: 42-48.



52.   M. Chrispeels, D. Sadava. (1971). Peptidyl proline hydroxylase in carrot disks. Biochem. Biophys. Acta   227: 278-287.



53.   D. Sadava, M. Chrispeels. (1971). Intracellular site of proline hydroxylation in plant cells. Biochemistry   10: 4290-4295.



54.   M. Chrispeels, D. Sadava. (1969). Cell wall protein in plants: autoradiographic evidence. Science   165: 299-300.



55.   D. Sadava, C. Miller. (1967). Taxonomy of last instar larval remains of Spilonota ocellana. Canad. Ent.   99: 436-442.



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