JSD seminar series


Plumbing the Cortex: Imaging Blood Flow in Capillaries in the Awake Mouse Brain
Patrick Drew
From: 11:00 AM To: 12:00 PM
Thursday, Dec 3, 2009
Burns Lecture Hall
Understanding the dynamics and regulation of blood flow in the brain is important as many brain imaging techniques, such as fMRI, use increased blood flow as an indirect measure of neural activity. Further, acute and chronic decreases in blood flow to the brain are at the root of many neurological diseases such as stroke and dementia. However, most studies of blood flow dynamics are performed in animals under anesthesia, which introduces artifacts by altering the neural and cardiovascular state of the animal. In order to understand blood flow and its regulation in the brain at its most fundamental level, I imaged the dynamics of blood flow in individual capillaries in the brains of awake mice. I will describe how I employed two-photon microscopy , which allows visualization of cellular level structures deep within the brain, to observe spontaneous, low frequency fluctuations in capillary velocity in the awake animal, and large responses to sensory stimulation. Lastly, I will talk about measurements of the spatial scale of blood flow correlations in cortical capillaries.
FMI: Contact Sam Tanenbaum
Seminar Registered by: Melissa Coleman


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